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Prof. Barry T. Rouse
Distinguished Professor
Biomedical and Diagnostic Sciences

Research Interests

  • My research is in the field of infectious disease and has focused on viral immunology and immunopathology. We have mainly studied herpes simplex virus (HSV) infection in mice both with a view to devising successful vaccines and more particularly to determine how HSV causes tissue damage in critical tissues such as the eye and nervous system. We have worked on both host innate and adaptive immune mechanisms, particularly the T cells responsible for immunity as well as those involved in orchestrating tissue damage. We have defined the role of several subtypes of proinflammatory T cells as well as numerous cytokines and chemokines. Our group was the first to show a role of regulatory T cells (Treg) in the host response to a virus infection. We demonstrated that Treg responses could act to inhibit the efficiency of immunity, but that Treg were valuable to modulate the severity of immunopathological responses, such as those that occur in the corneal stroma after HSV infection of the eye. My group has studied the important blinding lesion herpes stromal keratitis (SK) for >30 years and has demonstrated how multiple events set off by ocular HSV infection culminate in SK. We have paid particular attention to the non specific inflammatory cells, particularly neutrophils, that are recruited to the eye and which appear to be mainly responsible for the tissue damage that occurs. We were also the first to show a critical role for corneal neovascularization (CV) during SK pathogenesis, identified several angiogenic factors and studied how such factors are generated following HSV infection. We have also investigated how to diminish the extent of CV and have shown that controlling CV represents a valuable means of therapy, particularly if combined with approaches that control other critical events in lesion pathogenesis. Recently, we have also investigated various ways of modulating the extent of proinflammatory T cell involvement during SK with a view to understanding how to optimally achieve lesion resolution. We have shown that host derived factors such as Galectin 9 and Galectin 1 can be manipulated to achieve control of lesion severity as can be approaches that modulate Treg numbers and activity. We have also shown how molecules derived from Omega-3 polyunsaturated fatty acids, such as some synthetic resolvins and protectins, participate in the control of lesions. More recently we have focused on manipulating levels of multiple microRNA species specifically involved in SK as well as learning how to dampen changes in T cell function that contribute to tissue damage.

Education

  • University of Bristol, England -1965 - Bachelor of Veterinary Science with honors
  • University of Guelph, Canada -1967 - M.Sc.
  • University of Guelph, Canada - 1970 - Ph.D.
  • Walter and Eliza Hall Institute of Medical Research, Australia – 1970-72 – Postdoctoral research
  • University of Bristol, England - 1997 - D.Sc.

Teaching

  • Immunology

Funding

  • Two RO1 grants from National Institutes of Health

Professional Activities

  • Trained > 50 graduate students and postdoctoral fellows
  • Received several awards for research accomplishments most notably Alcon Award for outstanding contributions to vision research, 2000 and Medal of Merit, Warsaw University of Life Sciences, 2014.
  • Recipient of Fogarty Senior International Fellowship and Alexander Von Humboldt Fellowship
  • Been extensively involved in reviewing NIH grants since 1978 which includes 3 times as a permanent member of study section and once as chairman
  • Member of Faculty of 1000 since its inception
  • Coorganizer of several conferences
  • Member of editorial board and reviewer of many journals
  • Member of AAI and ASM for >30 years

Selected Publications out of > 400. Current H index 75

  • Bhela, S., Mulik, S., Reddy, P.B., Richardson, R.L., Gimenez, F., Rajasagi, N.K., Veiga-Parga, T., Rouse, B.T. 2014. Critical Role of MicroRNA-155 in Herpes Simplex Encephalitis. doi:10.4049/jimmunol.
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  • Veiga-Parga, T., Sehrawat, S., Rouse, B.T. 2013. Role of regulatory T cells during virus infection. Immunol Rev. 255: 182-96. PMCID: PMC3748387
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  • Rajasagi, N.K., Reddy, P.B., Mulik, S., Gjorstrup, P., Rouse, B.T. 2013. Neuroprotectin D1 reduces the severity of herpes simplex virus-induced corneal immunopathology. Invest Ophthalmol Vis Sci. 54: 6269-79. PMCID:PMC3776714
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  • Veiga-Parga T, Giménez F, Mulik S., Chiang EY, Grogan JL, Rouse BT. 2013. Controlling herpetic stromal keratitis by modulating lymphotoxin-alpha-mediated inflammatory pathways. Microbes Infect. 15: 677-87. PMCID: PMC3769451
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  • Gimenez, F., Suryawanshi, A., Rouse, B.T. 2013. Pathogenesis of Herpes stromal keratitis-a focus on corneal neovascularization. 2013. Progress in Retinal and Eye Research. 33: 1-9. PMCID: PMC3511644
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  • Mulik S Bhela,S , Rouse B.T. Potential function of miRNAs in herpetis stromal keratitis. 2013. Invest. Ophthalmol.Vis. Sci. 54: 563-73. PMCID: PMC3558297
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  • Veiga-Parga, T., Gimenez, F., Mulik, S. ,  Sharma, S., Sparwasser, T.,  Rouse, B. T. 2012  On the role of regulatory T cells during virus induced inflammatory lesions  J Immunol. 189.5924-5933. PMCID: PMC3518750
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  • Mulik, S., Xu, J., Reddy, P.B., Rajasagi, N.K., Gimenez, F., Sharma, S., Lu, P.Y., Rouse, B.T. 2012. Role of MicroRNA-132 in Angiogenesis after Ocular Infection with Herpes Simplex Virus. Am J Pathol. 181:525-534. PMCID: PMC3409434
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  • Reddy, P.B., Schreiber, T., Rajasagi, N.K., Suryawanshi, A., Mulik, S., Veiga-Parga, T., Niki, T., Hirashima, M., Podack, E.R., Rouse, B.T. 2012.TNFRSF25 agonistic antibody and galectin-9 combination therapy controls herpes simplex virus-induced immunoinflammatory lesions. J Virol. 86:10606-20. PMCID: PMC3457251
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  • Suryawanshi, A., Veiga-Parga, T., Reddy, P.B., Rajasagi, N.K, Rouse, B.T. 2012. IL-17A Differentially Regulates Corneal VEGF-A and sVEGFR-1 Expression and Promotes Corneal Angiogenesis after HSV Infection. J. Immunol. 7: 3434-46. PMCID: PMC3311700
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  • Rajasagi, N.K.,  Reddy, P.B., Suryawanshi, A., Mulik, S.,  Gjorstrup, P., Rouse, B.T. 2011 Controlling herpes  simplex virus induced ocular inflammatory lesions with the lipid derived mediator Resolvin E1. J Immunol.  186: 1735-1746. PMCID: PMC3888773
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  • Suryawanshi, A., Veiga-Parga, T., Rajasagi, N., Reddy, P.B., Sehrawat, S., Sharma, S., Rouse, B.T. 2011. Role of IL-17 and Th17 Cells in HSV Induced Corneal Immunopathology. J. Immunol.  187: 1919-30. PMCID: PMC3150378
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  • Sehrawat,S., Rouse, B.T. 2011.Tregs and infections: on the potential value of modifying their function. J Leukoc Biol. 90: 1079-87. PMCID: PMC3236550
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  • Veiga-Parga, T., Suryawanshi,,A., Rouse,,B.T. 2011. Controlling Immunoinflammatory viral lesions by modulating aryl hyrdrocarbon receptor signaling. Plos Pathogens. e1002427. PMCID: PMC3234248
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  • Suryawanshi, A., Mulik, S., Sharma, S., Reddy, P.B., Sehrawat, S., Rouse, B.T. 2011. Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.  J.Immunol. 186: 3653-3665. PMCID: PMC3892272
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  • Rouse,,B..T., Sehrawat, S. 2010 Immunity and immunopathology to viruses- what decides the outcome?  Nat Rev Immunol. 10: 514-526. PMCID: PMC3899649
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  • Sarangi, P., Rouse, B.T. 2010 Herpetic Keratitis in Ocular Diseases Mechanisms and Management. Ed by Levin, L.A., Albert,.D..M. Saunders Elsevier pp 91-97.
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  • Sehrawat S., Reddy, PB, Rajasag, N. K., Suryawansi, A., Hirashima, M., and Rouse, B.T. 2010. Galectin-9/TIM-3 interaction regulates virus-specific primary and memory CD8+ T cell responses. Plos Pathog. 6 e1000882. PMID: 20463811
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  • Sehrawat S., Suryawanshi, A., Hirashima M and Rouse, B.T. 2009. Role of TIM3/Galectin-9 inhibitory interaction in viral induced immunopathology: Shifting the Balance towards regulators. J. Immunol. 182: 3191-3201. PMID: 19234217.
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  • Sehrawat, S., Suryawanshi, A., Hirashima, M., Rouse, B.T. 2009. Role of Tim3/Galectin-9 inhibitory interaction in viral induced immunopathology: Shifting the Balance towards regulators. J.Immunol. 182: 3191-3201. PMCID: PMC3891207
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  • Sehrawat, S., Suvas, S., Sarangi, P., Suryawanshi, A., Rouse B.T. 2008. In vitro generated antigen-specific CD4+CD25+Foxp3+ regulatory T cells control the severity of virus induced immunoinflammatory lesions. J.Virol. 82: 6838-6851. PMCID: PMC2446952
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  • Sarangi P., Sehrawat, S., Suvas, S, Rouse, B.T. 2008. IL-10 and Natural regulatory T cells: Two independent anti-inflammatory mechanisms in HSV induced ocular immunopathology. J. Immunol.  180 6297. PMID: 18424753
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  • Rouse, B.T., Sarangi, P., and Suvas, S. 2006. Regulatory T cells in virus infections. Immun. Revs. 212: 272-289. PMID:16903920
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  • Belkaid, Y., and Rouse, B.T. 2005. Natural regulatory T cells in infectious disease Nature Immunol. 6: 353-360. PMID: 15785761
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  • Suvas, S., Kim, B.S., Azkur, K., Kumaraguru, U., and Rouse, B.T. 2004. CD4+ CD25+ regulatory T cells control the severity of viral immunoinflammatory lesions. J. Immun. 172:4123-4129. PMID: 15034024.
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  • Suvas, S., Kumaraguru, U., Pack, C. D., Lee, S., and Rouse, B. T. 2003. CD4+ CD25+ T cells regulate virus-specific primary and memory CD8+ T cell responses. J. Exp. Med. 198:889-901. PMID: 12975455
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